How to Maintain HPLC Columns

HPLC column maintenance is mandatory for the column performance and the column life, period!


A column is the heart of an HPLC system. No matter whether you are in pharma/biopharma, CRO/CMO/CDMO/CRAMS, lifesciences, academia or any other domain that leverages HPLC, you need to take care of the HPLC columns; else, your productivity, return of investment or even reputation may be jeopardized. While an HPLC column will be unusable in the long run, be prepared for bad chromatography and/or lower than expected number of injections if you fail to keep up an HPLC column as an analyst.


Welcome to an introduction to HPLC column maintenance and best practices by Chemaficionado.




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HPLC = High-performance liquid chromatography, CRO = Contract research organization, CMO = Contract manufacturing organization, CDMO = Contract development & manufacturing organization, CRAMS = Contract research and manufacturing services

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